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Biological Chemistry Mass Spec Facility

Because of changes to our instrumentation, not all users will have access to the qTOF instrument.  The old HPLC is being upgraded with an Agilent 1260 Infinite nanoLC with Chip Cube ion source which will improve chromatographic resolution while also reducing sample size.  Please, bear with us as we make this change.
HIM Building, Room 1005
  Instrumentation for this facility includes an Agilent 6520 Accurate-Mass quadrupole time-of-flight (Q-TOF) LC/MS system with great mass accuracy, mass resolution, and sensitivity for small molecule mass identification/quantitation and metabolomics experiments. It is equipped with an Agilent 1200 series HPLC with DAD UV/Vis detection for normal flow LC/MS and nitrogen CID for MS/MS. Instrument operation and data analysis (free for registered users) is performed in the Agilent MassHunter software suite (Qual, Quant, Profiler Professional).
 
Mass Spec Rates:
For Internal Harvard users:
(Funds managed through HMS)
  For outside Harvard users:
     
$50 new user training fee
$40/hr., for runs between 0-8 hrs.
$25/hr., for runs between 9-15hrs.
$15/hr., for runs greater than 15hrs.
  $57.37 new user training fee
$62.52/hr., regardless of duration
     
Documents and Authorization Forms
All users of the Mass Spec must complete the Biological Chemistry Mass Spec Facility User Authorization Form and return it by e-mail to mckay_wood@hms.harvard.edu before you can use the facility.
 
Biological Chemistry Mass Spec Facility User Authorization Form
BCMS Facility Rules of Use
Agilent 6520 LC/MS Instrument Operation Guidelines
     
Scheduling and Calendar Signup

Please sign up for times on the online calendar WalkerQTOF. Try to be as accurate as possible to avoid interfering with other users’ run times.
Do not sign up more than 2 weeks in advance. Please be courteous to other users when signing up for multiple times and during peak hours.
Users will be charged for scheduled time, even if runs are not performed. Users may agree with each other to substitute in for times, provided they share a billing code.
Scheduled runs can be canceled up to 24 hours in advance to avoid being charged.
     
Instrument Maintenance


Weekly maintenance is performed by the Walker Lab to maintain instrument integrity and sensitivity.
All users should immediately e-mail mckay_wood@hms.harvard.edu with instrument shutdown/critical errors.
All users will be notified immediately if the instrument requires servicing.  Users will not be charged for runs that could not be used due to instrument downtime.
An email will be sent when the instrument is operational again so that users may reschedule on the online calendar.

A week-long annual maintenance is scheduled during the summer and will be announced to users in advance.